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This is another reason why I like to use lash pads instead of just tape to secure the lashes. Some clients fall asleep and jump up and it just makes me feel more at ease knowing there is a little more padding there. Also, it is really important that you check the pressure that you are applying on the pad when you are holding your isolation in place. You may find while you are getting the hang of things that you apply too much pressure on the pad when you reach for your lash.
This can be dangerous as not only will this push down on the pad and expose the eye (the fumes can cause irritation and the repetitive action of the pad rubbing against the lid can cause soreness) but more importantly it will be uncomfortable and you don't want to stab your client in the eye! Your tweezers should just rest on the pad. Using a glue ring can help with this problem as your adhesive is more easily accessible to you but as it's right in front of you it is especially important that you wear a mask.
You can use straight or curved tweezers, whichever you find easier. Brush through the lashes first with your lash wand to separate them as much as possible and so you can see the direction they face. With your non-dominant hand, use the tweezers to slice into the lashes until you manage to isolate a single lash. Hold in place and with the tweezers in your dominant hand pick up a single synthetic lash.
I always isolate first and then dip the lash into the adhesive but when you get faster and more efficient at isolating you may choose to isolate after you have your synthetic lash ready. It all depends on how quickly you can isolate and how fast setting your adhesive is.
Isolation can be a little tricky at first but will become second nature with a bit of practice. During your training you should have been shown how to hold the tweezers but here's a quick run through. I now find the thinner and sharper the point on the tweezer, the better I can isolate.